The Validator provides signal quality testing for coax, telephone, audio and security cables in the installation and maintenance of local premise networks. Each run consisted of all six capillaries filled with 10 μL streptavidin, and for each system, 3 replicate runs were performed. The Validator NT950 offers active network testing, digital performance and speed certification for CAT5, CAT5e, and CAT6 Ethernet cables. A reference protein, streptavidin at a concentration of 1 mg/mL, was measured on 13 different Tycho systems over a period of two weeks. In this study we demonstrate the outstanding repeatability and intermediate precision of measurements with Tycho for both raw data and analyzed results. It records these unfolding profiles so you can study your protein in real-time or use the data as a reference to compare and validate the quality of your sample to any future batches. Tycho generates thermal unfolding profiles and identifies the inflection temperature (T i) that represent unfolding transition(s) or discrete changes in a protein’s structural integrity. The system measures the fluorescence of instrinsic tryptophan and tyrosine residues detected at both 350 nm and 330 nm as a specific defined temperature ramp is applied. It does this all by examining your protein’s structural integrity or foldedness in a label-free way. Tycho TM NT.6 tells you so much about the quality of your protein - presence, purity, concentration, functionality and similarity - in a single experiment.

Validator-nt software